The microFAST Isotope combines the advantages of the Micro DX autosampler with single or dual loop FAST capabilities and syringe based sample loading/injection. The system accurately loads a loop and then smoothly injects the solution into a μFlow concentric nebulizer at defined rates from 5-1000 μL/min. The valve on the flow injection system selects from two discrete, parallel flow paths. This allows rapid switching between solutions with minimal dead volume between the valve and the nebulizer.
The microFAST Isotope utilizes a syringes to precisely load micro-volumes samples (as low as 5 µL) and inject them at any user defined flow rate. The figures below illustrate the capability to produce smooth injection profiles for 5 µL samples at 100 µL/min with excellent reproducibility (<2% RSD, n=10).
The microFAST Isotope precisely syringe injects micro-volume samples (5 µL) into the Apex Omega at 5 µL/min, 10 µL/min and 20 µL/min. The figures below plot signal profiles of approximately 60 sec, 30 sec and 15 sec for a 5 µl sample. Increased sensitivity with flow rate is a result of high sample transport efficiency using the Apex Omega. Smooth injection profiles for 5 µL samples at all flow rates, results in excellent reproducibility (<2% RSD, n=5).
The dual loop configuration one loop is cleaned and loaded with sample, while the other loop is injected and analyzed. Rapid switching between loops drastically reduces long uptake and wash times associated with low sample flow rate.
Dual Loop Patent US 8,925,375 B1 on January 6, 2015