prepFAST IC - Automated Speciation

Automatically determine total metal concentrations or speciate your sample with prepFAST IC. By performing automated inline dilutions immediately before separation, the system eliminates potential species conversions, providing fast, accurate, and reproducible separations.

The prepFAST autodilution alternative, when combined with syringe driven speciation, provides: 1) rapid fully automated analysis of species; 2) autocalibration for any speciation system; 3) autodilution of samples to obtain reproducible separations and elution/retention times and; 4) accurate and precise results.

Features:

  • Auto switching between speciation and total metals
  • Autocalibrate
    • Total metals
    • Speciation
  • Autodilute
    • Total metals
    • Speciation
  • Gradient elution syringe pump
  • Automated tuning
  • Syringe loading
    • Viscous samples

prepFAST IC
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Chromium Speciation in Drinking Water

Chromium exists in the environment in several forms which differ in their effects upon organisms. Chromium enters the air, water and soil as Cr(III) and Cr(VI) through natural processes and human activities. At certain levels, Cr(III) is an essential nutrient for humans. On the other hand, Cr(VI) is detrimental to human health and is considered carcinogenic.

For this reason, there is an interest in knowing the level of Cr(VI) in drinking water. Elemental Scientific’s prepFAST IC is a robust, speciation system that utilizes autocalibration and a low-pressure anion exchange column to separate, detect, and quantify Cr(III) and Cr(VI) species in water samples.


Cr in Drinking Water Chromatogram

Cr in Drinking Water Chromatogram

Cr(VI) Speciation Calibration Curve

Cr(VI) Speciation Calibration Curve


Arsenic Speciation

Farming in areas with arsenic enriched soil and groundwater can lead to an increase in arsenic levels found in several dietary staples. Naturally occurring organic and inorganic arsenic are composed of several species with a wide range of toxicity levels. Inorganic As (III and V) is toxic at just 10mg/kg Lethal Dose (LD), while the organic forms (AsB, DMA and MMA) are much less toxic (LD50 >700 mg/kg). It is therefore important to know the species of As that compose the total As found in a wide variety of food products.

As Speciation


As Autocalibration Chromatogram

As Calibration Chromatogram

As(III) Speciation Calibration Curve

As(III) Speciation Calibration Curve

2 2 2 2

Chromium Speciation in Toys

The regulation (EN 71-3 and 71-3A1) concerning toy safety requires the detection of Cr VI at very low levels, possibly in the presence of a large excess of Cr III. In this new regulation the maximum allowed value in Category II toys for Cr VI is 5µg/Kg. This means that after sample preparation and extraction with 0,07M HCl for 2 hours, the measured concentration of Cr VI must be less than 10ng/L. The typical method used to separate Cr III from Cr VI is HPLC-ICP-MS, however detection of such low levels can be a challenge for this technique. The principal issues are column capacity, potential contamination in the sample preparation steps, and the ArC+ interference on 52Cr+. In this paper we examine the advantages of low pressure ion exchange chromatography coupled to an ICPMS fitted with a universal cell (collision/reaction) to perform low level Cr speciation. The analysis is fully automated by using a syringe-driven inline dilution system to automatically calibrate the ICPMS for both Cr III and Cr VI. To validate the method real samples have been measured and spiked to the lowest level required by the new regulations.

Cr Speciation Proof

Cr Chromatogram

Cr Autocalibration Chromatogram

Cr(VI) Speciation Calibration Curve

Cr(VI) Speciation Calibration Curve


Mercury Speciation

Mercury (Hg) exists in the environment in several forms, all of which are toxic to humans to some degree. Obtaining precise, timely measurements of Hg in environmental samples—especially methylmercury (MeHg) in water samples—is essential to monitoring potential toxicity issues. However, detecting trace amounts of the element is an obstacle that can prevent accurate analysis of Hg and MeHg levels.

Mercury Spike Recovery Study with Fish Tissue

Mercury Spike Recovery Study with Fish Tissue

Hg Speciation

Hg Speciation


Selenium Speciation

Selenium is a naturally occurring element that is widely distributed throughout the environment and has many industrial applications such as a component in the manufacture of photocells, rectifiers, metal alloys and medical therapeutic agents, as well as an oxidizing agent in drug and chemical manufacture. Selenium is usually found as a compound with other elements such as copper, silver, lead and nickel and the mining/refining processes for these elements yields a buildup of selenium residue through accidental mobilization. Certain species of selenium have greater toxic effects in higher concentrations, so determination of individual species is of critical importance.

Selenium in Drinking Water

Selenium in Drinking Water

Se Speciation

Se Speciation Proof



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